منابع مشابه
Low Pressure Microfluidic-based Dna Fragmentation
We report a low-pressure microfluidic deoxyribonucleic acid (DNA) fragmentation device based on a combination of mechanical hydrodynamic shearing and low temperature sample heating. Conventional DNA fragmentation based on hydrodynamic shearing is capable of achieving fragment lengths (FL) < 10k bp with pressures ∆P > 1 bar. The application of a small amount of additional thermal energy to the b...
متن کاملCell nucleus and DNA fragmentation are not required for apoptosis
Apoptosis is the predominant form of cell death and occurs under a variety of physiological and pathological conditions. Cells undergoing apoptotic cell death reveal a characteristic sequence of cytological alterations including membrane blebbing and nuclear and cytoplasmic condensation. Activation of an endonuclease which cleaves genomic DNA into internucleosomal DNA fragments is considered to...
متن کامل[Letter to the Editor] DNA Fragmentation Is Not Associated with Apoptosis in Zerumbone-induced HepG2 Cells
Zerumbone is a cytotoxic compound isolated from the herbal plant, Zingiber zerumbet Smith, which exhibits antitumor activity [1-2], anti-inflammatory effects and possesses anti-proliferative potentials in a variety of cell lines [3-4]. DNA fragmentation indicates an early event of apoptosis leading to cell death due to the absence of new cellular proteins synthesizing for cell survival. Previou...
متن کاملMonochloramine induced DNA fragmentation in gastric cell line MKN45.
Monochloramine (NH2Cl) is known to be one of the virulence factors in Helicobacter pylori-associated gastric mucosal injury. The present study was designed to examine NH2Cl-evoked DNA fragmentation in the gastric epithelial cell line MKN45. NH2Cl was produced by mixing NH3 with sodium hypochlorite (NaClO). MKN45 cells were exposed to NH2Cl, NH3, or NaClO in Hanks' balanced salt solution. DNA cl...
متن کاملDNA fragmentation induced by ionizing radiation - AFM study
DNA double-strand breaks (DSBs) produced by ionizing radiation are considered to be particularly malignant DNA lesions, with experimental evidence suggesting that misrepaired or unrepaired DSBs are the lesions leading to cell killing, mutations or carcinogenesis [1, 2]. DSBs production is determined by the spatial distribution of ionization events, dependent on the physical properties of the ra...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2011
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-010-9525-1